Dr Gaetan Burgio: Any Idiot can do it’: An overview of the CRISPR gene editing technology from basic biology to irreproducible research
Location:Children's Medical Research Institute Seminar Room
Dr Gaetan Burgio, Group Leader and Head of the Transgenesis Facility, John Curtain School of Medical Research
Novel precision genetic technologies such as CRISPR/Cas9 genome editing technology offer novel avenues to a better understanding the mechanisms of diseases. Using CRISPR/Cas9 we are able to precisely modify the mouse or the human genome by creating knockout or a specific single nucleotide change to enable the study of the function of the gene of interest. The generation of these models lies on the ability of Cas9 to create a double strand break in the DNA and the repair to occur via the error prone Non-Homologous End Joining (NHEJ) or the precise Homology direct Repair (HDR) mechanisms. A large body of work has been recently dedicated to either improve the technology to generate efficiently knockout or knock-in mouse models (point mutations, tags or floxed alleles) or to cure diseases. The rapid pace of the technology development has generated a lot of excitement but also some disappointment over the lack of reproducibility of the experiments. This led to a substantial loss of research time and funding. This short presentation will give an overview over the basic biology questions to the application of the CRISPR technology to interrogate diseases. I will also discuss the initiatives undertaken to tackle irreproducible research and how to address these problems in the field of gene editing technology.
Host: Leszek Lisowski