RNAi and CRISPR Seminar
Presented by Dharmacon
Location:Centenary Institute, Level 6 Lecture Theatre
RNAi has long been used for functional genomics screening in cell-based assays. CRISPR is newer to this application, but showing promise as a complimentary, and possibly more robust mechanism for loss-of function screens. Whether you are interested in screening a handful of genes, a pathway, or a whole genome, there are several options to consider.
In this seminar we will discuss the various pooled and arrayed options for screens of small gene sets or whole genome collections. In addition to examples of successful assays, we will also present important considerations for your screening workflows and how to choose the best tools for your needs.
Presented by: Doris Beylkin, Field Application & Support Scientist, Dharmacon
Doris Beylkin earned her PhD in Molecular, Cellular, and Developmental Biology from the University of Colorado in Boulder. She has been providing scientific support for all Dharmacon products, including CRISPR-Cas9 and RNAi technologies, since 2006. During this time she has also worked closely with members of the Genomics Discovery Initiative (formerly the RNAi Global Initiative), an alliance of international biomedical researchers using genome-wide RNAi and CRISPR libraries for high throughput screening. This group aims to accelerate scientific discovery through collaboration, data sharing, and development of best practices.