Sydney West Translational Cancer Research Centre
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Roland Gamsjaeger: The structural and molecular details of the involvement of single-stranded DNA binding proteins hSSB1 and hSSB2 in DNA damage repair processes

CMRI Seminar

Aug
9
12
30
P
1
30
P

Location:

Seminar Room 1 and 2, Children’s Medical Research Institute (CMRI), 214 Hawkesbury Rd, Westmead

Roland Gamsjaeger, Senior Lecturer, Western Sydney University

Seminar Title: The structural and molecular details of the involvement of single-stranded DNA binding proteins hSSB1 and hSSB2 in DNA damage repair processes

Host: Tony Cesare

Abstract: Single-stranded DNA-binding proteins (SSBs) are required for all known DNA metabolic events such as DNA replication, recombination and repair. Over the course of the last 10 years, two novel SSBs (hSSB1 and hSSB2) that are distinct to the other well-known SSB in humans, Replication Protein A (RPA), have been discovered and shown to be essential in several important DNA repair processes. Both SSBs possess a well-structured OB (oligonucleotide/oligosaccharide-binding) domain that is able to recognise single-stranded DNA (ssDNA) followed by a flexible carboxyl-tail implicated in the interaction with other proteins. This talk will focus on the structural characterisation of hSSB1 and hSSB2 involvement in DNA repair using a combination of biophysical and structural methods. The molecular details of the binding of both SSBs to DNA that vary depending on the exact DNA repair pathway have been determined and will be presented. Given that targeting DNA repair pathways has been a widely used strategy for the development of novel cancer drugs for many years, a thorough understanding of these processes is an important prerequisite for the future designs of tailored SSB inhibitors.